Glycerol-mediated improvement of heterologous aurachin D production in E. coli
to its potent antibiotic and antiprotozoal properties. The biosynthesis of aurachin D from 2-methyl-1 H -quinolin-4-one (MQO) is catalyzed by the membrane-bound farnesyltransferase AuaA. Recently, this [...] reconstructed in the heterologous host Escherichia coli , yielding an aurachin D titer of 17 mg L −1 . In this study, we investigated the influence of the medium on aurachin D biosynthesis in E. coli in [...] minimal medium and adjustment of the MQO precursor concentration, an aurachin D titer of 124 mg L −1 was achieved in a microbioreactor system, highlighting the superior performance of minimal over complex …
